Soybean cultivar S06-01JR122235

ABSTRACT

The present invention is in the field of soybean variety S06-01JR122235 breeding and development. The present invention particularly relates to the soybean variety S06-01JR122235 and its progeny, and methods of making S06-01JR122235.

THE FIELD OF THE INVENTION

The present invention is in the field of soybean variety S06-01JR122235breeding and development. The present invention particularly relates tothe soybean variety S06-01JR122235 and its progeny, and methods ofmaking.

BACKGROUND OF THE INVENTION

Soybean Glycine max (L) is an important oil seed crop and a valuablefield crop. However, it began as a wild plant. This plant and a numberof other plants have been developed into valuable agricultural cropsthrough years of breeding and development. The pace of the developmentof soybeans, into an animal foodstuff and as an oil seed hasdramatically increased in the last one hundred years. Planned programsof soybean breeding have increased the growth, yield and environmentalhardiness of the soybean germplasm.

Due to the sexual reproduction traits of the soybean, the plant isbasically self-pollinating. A self-pollinating plant permits pollen fromone flower to be transferred to the same or another flower of the sameplant. Cross-pollination occurs when the flower is pollinated withpollen from a different plant; however, soybean cross-pollination is arare occurrence in nature.

Thus the growth and development of new soybean germplasm requiresintervention by the breeder into the pollination of the soybean. Thebreeders' methods of intervening in the pollination depend on the typeof trait that is being bred. Soybeans are developed for a number ofdifferent types of traits morphological (form and structure),phenotypical, or for traits like growth, day length, temperaturerequirements, initiation date of floral or reproductive development,fatty acid contents, insect resistance, nematode resistance, herbicideresistance and yield. The genetic complexity of the trait often drivesthe selection of the breeding method.

Due to the number of genes within each chromosome, millions of geneticcombinations exist in the breeders' experimental soybean material. Thisgenetic diversity is so vast that a breeder cannot produce the same twocultivars twice using the exact same starting parental material. Thus,developing a single variety of useful commercial soybean germplasm ishighly unpredictable, and requires intensive research and development.

The development of new soybeans comes through breeding techniques, suchas: recurrent selection, mass selections, backcrossing, single seeddescent and multiple seed procedure. Additionally, markers' assistedbreeding allows more accurate movement of desired alleles or evenspecific genes or sections of chromosomes to be moved within thegermplasm that the breeder is developing. RFLP, RAPD, AFLP, SSR, SNP,SCAR, isozymes, are all forms of markers that can be employed inbreeding soybeans or in moving traits into soybean germplasm. Otherbreeding methods are known and are described in various soybeantextbooks.

When a soybean variety is being employed to develop a new soybeanvariety or an improved variety the selection methods includebackcrossing, pedigree breeding, recurrent selection, modified selectionand mass selection. The efficiency of the breeding procedure along withthe goal of the breeding are the factors for determining which selectiontechniques are employed. A breeder continuously evaluates the success ofthe breeding program and therefore the efficiency of any breedingprocedure. The success is usually measured by yield increase, commercialappeal and environmental adaptability of the developed germplasm.

The development of new soybean cultivars most often requires thedevelopment of hybrid crosses (some exceptions being initial developmentof mutants directly through the use of the mutating agent, certainmaterials introgressed by markers, or transformants made directlythrough transformation methods) and the selection of progeny therefrom.Hybrids can be achieved by manual manipulation of the sexual organs ofthe soybean or by the use of male sterility systems. Breeders often tryto identify true hybrids by a readily identifiable trait or the visualdifferences between Inbred and hybrid material. These heterozygoushybrids are then selected and repeatedly selfed and reselected to formnew homozygous soybean lines.

Mass and recurrent selection can be used to improve populations. Severalparents are intercrossed and plants are selected based on selectedcharacteristics like superiority or excellent progeny. Outcrossing to anumber of different parents creates fairly heterozygous breedingpopulations.

Pedigree breeding is commonly used with two parents that possessfavorable, complementary traits. The parents are crossed to form a F1hybrid. The progeny of the F1 hybrid is selected and the best individualF2s are selected; this selection process is repeated in the F3 and F4generations. The inbreeding is carried forward and at F5-F7 the bestlines are selected and tested in the development stage for potentialusefulness in a selected geographic area.

In backcross breeding a genetic allele or loci is transferred into adesirable homozygous recurrent parent. The trait is in the donor parentand is tracked into the recurrent parent. The resultant plant is likethe recurrent parent with the new desired allele or loci.

The single-seed descent method involves use of a segregating plantpopulation for harvest of one seed per plant. Each seed sample isplanted and the next generation is formed. When the F2 lines areadvanced to F6 each plant will be derived from a different F2. Thepopulation will decline due to failure of some seeds, so not all F2plants will be represented in the progeny.

New varieties must be tested thoroughly to compare their developmentwith commercially available soybeans. This testing usually requires atleast two years and up to six years of comparisons with other commercialsoybeans. Varieties that lack the entire desirable package of traits canbe used as parents in new populations for further selection or aresimply discarded. The breeding and associated testing process is 8 to 12years' of work prior to development of a new variety. Thousands ofvarietal lines are produced but only a few lines are selected in eachstep of the process. Thus the breeding system is like a funnel withnumerous lines and selections in the first few years and fewer and fewerlines in the middle years until one line is selected for the finaldevelopment testing.

The selected line or variety will be evaluated for its growth,development and yield. These traits of a soybean are a result of thevariety's genetic potential interacting with its environment. Allvarieties have a maximum yield potential that is predetermined by itsgenetics. This hypothetical potential for yield is only obtained whenthe environmental conditions are perfect. Since perfect growthconditions do not exist, field experimentation is necessary to providethe environmental influence and to measure its effect on the developmentand yield of the soybean. The breeder attempts to select for goodsoybean yield potential under a number of different environmentalconditions.

Selecting for good soybean yield potential in different environmentalconditions is a process that requires planning based on the analysis ofdata in a number of seasons. Identification of the varieties carrying asuperior combination of traits, which will give consistent yieldpotential, is a complex science. The desirable genotypic traits in thevariety can often be masked by other plant traits, unusual weatherpatterns, diseases, and insect damage. One widely employed method ofidentifying a superior plant with such genotypic traits is to observeits performance relative to commercial and experimental plants inreplicated studies. These types of studies give more certainty to thegenetic potential and usefulness of the plant across a number ofenvironments.

In summary, the goal of the soybean plant breeder is to produce new andunique soybeans and progeny of the soybeans for farmers' commercial cropproduction. To accomplish this the plant breeder painstakingly crossestwo or more varieties or germplasm. Then the results of this cross arerepeatedly selfed or backcrossed to produce new genetic patterns. Neweravenues for producing new and unique genetic alleles in soybeans includeintroducing mutations or transgenes into the genetic material of thesoybean are now in practice in the breeding industry. These geneticalleles can alter pest resistance such as insect resistance, nematoderesistance, herbicide resistance, or they can alter the plant's diseasetolerance, or its fatty acid compositions, the amount of oil produced,and/or the amino acid compositions of the soybean plant or its seed.

The traits a breeder selects for when developing new soybeans are drivenby the ultimate goal of the end user of the product. Thus if the goal ofthe end user is to resist a certain plant disease so overall more yieldis achieved, then the breeder drives the introduction of genetic allelesand their selection based on disease resistant levels shown by theplant. On the other hand, if the goal is to produce specific oil, with ahigh level of oleic acid and a lower level of linoleic acid, then thebreeder may drive the selection of genetic alleles based on levels offatty acids in the seed and accept some lesser yield potentials or otherless desirable agronomic traits.

The new genetic alleles being introduced in to soybeans are widening thepotential uses and markets for the various products and by-products ofthe oil from the seed plants such as soybean. A major product extractedfrom soybeans is the oil in the seed. Soybean oil is employed in anumber of retail products such as cooking oil, baked goods, margarinesand the like. Another useful product is soybean meal, which is acomponent of many foods and animal feedstuffs.

SUMMARY OF THE INVENTION

One embodiment of the invention relates to seed of a soybean cultivardesignated S06-01JR122235. The invention relates to the plant from theseed designated S06-01JR122235, or the plant parts. The invention alsoencompasses a tissue culture of regenerable cells, cells or protoplastsbeing from a tissue selected from the group consisting of: leaves,pollen, embryos, meristematic cells, roots, root tips, anthers, flowers,ovule, seeds, stems, pods, petals and the cells thereof.

The invention in one aspect covers a soybean plant, or parts thereof,having all of the physiological and morphological characteristics of thesoybean plant.

Another aspect of this invention is the soybean plant seed or derivedprogeny which contains a transgene which provides herbicide resistance,insect resistance, resistance to disease, resistance to nematodes, malesterility, or which alters the oil profiles, the fatty acid profiles,the amino acids profiles or other nutritional qualities of the seed.

The present invention further covers a method for producing a soybeanseed with the steps of crossing at least two parent soybean plants andharvesting the hybrid soybean seed, wherein at least one parent soybeanplant is the present invention. In another aspect of the inventioncovers the hybrid soybean seed and the progeny soybean plant andresultant seed, or parts thereof from the hybrid seed or plant or itsprogeny.

In an additional aspect, the invention covers a method for producing asoybean progeny from the invention by crossing soybean lineS06-01JR122235 with a second soybean plant to yield progeny soybean seedand then growing progeny soybean seed to develop a derived soybean line.

Yet another aspect of the invention covers a method for a breedingprogram using plant breeding techniques which employ the soybean plantS06-01JR122235 as plant breeding material and performing breeding byselection techniques, backcrossing, pedigree breeding, marker enhancedselection, mutation and transformation.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows the Geographic Segment Chart—GSEGC shows the breakout forgrain yield at standard moisture for S06-01JR122235 across geographiclocations.

FIG. 2 shows the Group Mean chart (GRP_MN=Group Mean) of Grain Yield atstandard moisture for S06-01JR122235. This chart shows YieldStability−Win>5% of trial mean, Tie+ or −5% of trial mean, Loses<5% oftrial mean. The chart's vertical axis=yield of target variety, itshorizontal axis=location average yield. When the target variety line isabove the location average line this is desirable. The RSQ of the targetvariety shows a number. This number when it is closest to 1=yieldstability.

DETAILED DESCRIPTION

The following data is used to describe and enable the present soybeaninvention.

Trait Code Performance Trait Description VHNO Variety/Hybrid NumberYGSMN Grain Yield at Std MST (standard moisture) - YGSMN MRTYN MaturityDays from planting MRTYN HLDGR Harvest Lodging HLDGR PLHTN Plant Height(cm) PRR_R Phytophthora Root Rot To PRR_R IC_R Iron Chlorosis IC_R SDS_RSudden Death Syndrome SDS_R SCL_R Sclerotinia White Mold SCL_R FELSRFrogeye Leaf Spot FELSR STR_R Shattering STR_R GLDGR Green Lodging GLDGRPLBRR Plant Branching PLBRR EMRGR Emergence EMRGR

Syngenta Soybean Data Collection Traits and Timing Order 1 = Seedling, 2= Vegetative, 3 = Reproductive to grain-fill, 4 = Maturity Opportunisticratings should only be collected if differences occur. At least onevariety has a rating of 5 or greater and there is a spread of 3 in theratings. Trait Order Group Code Description Timing Comments # Loc Stage4 # Loc Stage 5 # Loc Stage 6 Scale 1 HS RRG_R Presence of RR geneV2-V4, 4-7 DAYS Home Loc's Home Loc's Home Locs's Roundup 1 = R, 5 =seg, AFTER SPRAYING 9 = susc, 1 HS RUR_R Roundup Tolerance YellowingR1-R2 Home Loc's Home Loc's Home Loc's 1-9 scale, 1 = no yellowing, 9 isextreme yellowing 1 OTH EMRGR Emergence - stand VE-V3 NK EPA_R = V2-V6,2 4 1 to 9 (1 = best) establishment, uniformity and 2005 on use EMRGRvigor 2 DIS IC_R Iron Deficiency Chlorosis August Internal Field MG00-2LMG00-4.9 MG00-4.9 1 to 9 (1 = best) Nursery 2 DIS ICFLR Iron DeficiencyChlorosis Initial flash V2 Internal Field MG00-2L MG00-4.9 MG00-4.9 1 to9 (1 = best) Yellow Flash June-July Nursery 2 DIS ICR_R Iron DeficiencyChlorosis 2-3 weeks after Internal Field MG00-2L MG00-4.9 MG00-4.9 1 to9 (1 = best) Recovery, (Stunting and/or ICFLR Nursery Green-up) 2 DISPRR_R Phytophthora Root Rot Scheduled Internal Field St. Joe St. Joe 1to 9 (1 = best) for field Tolerance in pond Nursery tolerance 2 NEMSCN_R SCN Field Rating June Internal Field Conesville, St. Conesville,St. Field = 1-9 (1 best) Nursery Joe Joe 3 AGR FL_CT Flower Color R1Confirmed Text for VSR Home Loc's Home Loc's Home Loc's W = White; P =Purple; Seg = Mix 3 AGR FL_CR Flower Color Rating R1 Confirmed Numericfor EDC Home Loc's Home Loc's Home Loc's 1 = White; 2 = Purple; 9 = Seg3 AGR PLCNR Plant canopy July-August (R1- Growth expressiveOpportunistic Opportunistic 1 to 9, 1 = Slender R6) locations. 5 =Intermediate, 9 = Bush 3 DIS ARSTR Asian Rust Rating When differencesOpportunistic Opportunistic Opportunistic 1 to 9 (1 = best) occur 3 DISBSR_R Brown Stem Rot When differences Opportunistic OpportunisticOpportunistic 1 to 9 (1 = best) occur 3 DIS CR_R Charcoal Rot Whendifferences Opportunistic Opportunistic Opportunistic 1 to 9 (1 = best)occur 3 DIS DPC_R Stem Canker (Northern) When differences OpportunisticOpportunistic Opportunistic 1 to 9 (1 = best) occur 3 DIS DPM_R StemCanker (Southern) When differences Opportunistic OpportunisticOpportunistic 1 to 9 (1 = best) occur 3 DIS FELSR Frogeye Leaf Spot Whendifferences Opportunistic Opportunistic Opportunistic 1 to 9 (1 = best)occur 3 DIS SCL_R Sclerotinia White Mold When differences OpportunisticOpportunistic Opportunistic 1 to 9 (1 = best) occur 3 DIS SDS_R SuddenDeath Syndrome When differences Opportunistic OpportunisticOpportunistic 1 to 9 (1 = best) occur 3 PERF GLDGR Green lodging R5 toR6 Where differences Opportunistic Opportunistic Opportunistic 1 to 9 (1best) 1 = All erect; 5 = occur 45 degrees; 9 = flat 4 AGR MRTYD MaturityDate (MMDD) - 95% of R8 Home locations + 1- ~1-2 ~5 ~10 MMDD (Sep. 10 =0910) plants in row have mature pod 2 off site/station color 4 AGR HILCTHilum Color R8 Text Home Loc's Home Loc's Home Loc's G = Gray, BR =Brown; BF = Buff; BL = Black; IB = Imperfect Black; Y = Yellow; IY =Imperfect Yellow; Seg = Mix 4 AGR PB_CR Pubescence Color R8-HarvestNumeric for EDC Home Loc's Home Loc's Home Loc's 1 = Gray; 2 = Tawny; 4= Lt. Tawny; 9 = Seg 4 AGR PB_CT Pubescence Color R8-Harvest Text forVSR Home Loc's Home Loc's Home Loc's G = Gray; T = Tawny; LT = Lt.Tawny; Seg = Mix 4 AGR PD_CR Pod Color R8-Harvest Numeric for EDC HomeLoc's Home Loc's Home Loc's 1 = Tan; 2 = Brown; 9 = Seg 4 AGR PD_CT PodColor R8-Harvest Text for VSR Home Loc's Home Loc's Home Loc's T = Tan;B = Brown; Seg = Mix 4 AGR PLBRR Plant branching R8-Harvest Growthexpressive Optional Home Loc's Home Loc's 1 to 9, 1 = no branching; 5 =locations. average branching; 9 = profuse branching 4 AGR PLHTN PlantHeight (cm) Harvest Growth expressive Optional Home Loc's Home Loc'sTaken in cm locations. 4 DIS GS_R Green Stem Harvest Where differencesOpportunistic Opportunistic Opportunistic 1 to 9 (1 = best) occur 4 PERFHLDGR Harvest Lodging R8-Harvest Where differences OpportunisticOpportunistic Opportunistic 1 to 9 (1 best) 1 = All erect; 5 = occur 45degrees; 9 = flat 4 PERF HVAPR Harvest Appearance Harvest Wheredifferences Opportunistic Opportunistic Opportunistic 1 to 9, 1 =Excellent, occur 5 = Avg, 9 = Poor 4 PERF STR_R Shattering Harvest Hillplot planter, Opportunistic + Opportunistic + 1 to 9 (1 = best) 2006?Bay and St. Joe Bay and St. Joe Roundup is a trademark of Monsanto TraitDefinitions Opportunistic Ratings developed in YT. Opportunistic ratingsshould only be collected if differences occur. At least one variety hasa rating of 5 or greater and there is a spread of 3 in the ratings.(Scale 1-9, 1 = Best). Emergence (EMRGR) A rating of the uniformestablishment and growth of seedlings. Taken from V1-V3, (Scale 1-9).Maturity (MRTYD) The month and day (MMDD) when 95% of the main stem podsin the plot have reached their mature color. Plant Height (PLHTN) Theaverage measured plant height in cm. Branching (PLBRR) Rating of thenumber of branches and their relative importance to yield. Taken atgrowth expressive locations. (Scale: 1-9, 1 = stick, no branching, 3 = 1seed bearing branch, 5 = average branching or 2-3 seed bearing branches,7 = 3-4 seed bearing branches, 9 = profuse branching). Green Lodging(GLDGR) Rating based on the average of plants leaning from vertical inR5 to R6 stage (Scale 1-9, 1 to 9 (1 best) 1 = All erect; 5 = 45degrees; 9 = flat). Harvest Lodging (HLDGR) Rating based on the averageof plants leaning from vertical at harvest (scale 1-9, 1 to 9 (1 best) 1= All erect; 5 = 45 degrees; 9 = flat). Shatter (STR_R) Rating ofpre-harvest loses based on amount of plants with open pods (Scale 1-9).Iron Deficiency Chlorosis (IC_R) final rating = average of initialYellow Flash (ICFLR) and recovery (ICR_R) taken 2-3 weeks after initialyellow flash. (Scale 1-9, 1 = Best). LS means analysis unequal entriesand reps between years. Soybean Cyst Nematode (CN_1R, CN_3R, CN_5R,CN14R) Greenhouse screen-30 day screen using infested soil. Rating Scalebased upon female reproduction index on a susceptible check set where<10% = R; <30% = MR; <60% = MS; >60% = S. In priority order, the racesscreened include: 3, 14, 1 & 5. Phytophthora Root Rot Field tolerance(PRR_R) or actual gene (RPS_T). Sudden Death Syndrome (SDS_R) based onleaf area affected, scale 1-9. Can be GH or field. Brown Stem Rot(BSR_R) Greenhouse pot-root dip or field rating of leaf symptoms. (Scale1-9) Root Knot Nematode Arenaria (MA_R), Incognita (MI_R), Javanica(MJ_R) RKN). Scale 1-9. Stem Canker North (DPM_R) Southern (DPM_R).Scale 1-9. Sulfentrazone (SUL_R) Greenhouse nursery rating damage ofmultiple rates. Scale 1-9. Metributzin (MET_R) Greenhouse nursery ratingdamage of multiple rates. Scale 1-9. Hypocotyl Elongation (HYP_R) Arating of a variety's hypocotyl extension after germination when plantedat 5″ depth in sand and maintained a warm germination environment for 10days. (Scale 1 = Long, 5 = Intermediate, 9 = Short)

Trait Definitions

Hypocotyl Elongation (HYPO) A rating of a variety's hypocotyl extensionafter germination when planted at a 5″ depth in sand and maintained awarm germination environment for 10 days.

Seedling Establishment (EMG) A rating of the uniform establishment andgrowth of seedlings.

Peroxidase Activity (Perox)—seed protein peroxidase activity is definedas a chemical taxonomic technique to separate cultivars based on thepresence or absence of the peroxidase enzyme in the seed coat. Ratingsare POS=positive for peroxidase enzyme or NEG=negative for peroxidaseenzyme.Plant Height (PLTHT) The average measured plant height in centimeters.Branching (BRANCH) Rating of the number of branches and their relativeimportance to yield. This rating is taken at growth expressivelocations.Green Lodging (GLODGE) Rating based on the average of plants leaningfrom vertical in R5 to R6 stage prior to maturity.Harvest Lodging (LODGE) Rating based on the average of plants leaningfrom vertical at harvest. Lodging score (1=completely upright,9=completely prostrate),Phytophthora Root Rot (PGR) or (PFT) Greenhouse pot—root dip method forPFT and hypodermic needle method for rating PGR.Root Knot Nematode (RKN) Greenhouse screen—45 day screen root inoculatedwith eggs and juveniles. Rating Scale based upon female reproductionindex on a susceptible check set determined by number of galls present.Stem Canker (STC) Based on number of lesions, scale 1-5.Sulfentrazone (SULF) Authority™ (commercial herbicide) Greenhousenursery rating damage of multiple rates.Metributzin (MET) Greenhouse nursery rating damage of multiple rates.Brown Stem Rot (BSR or BSR_R) This disease is caused by the fungusPhialophora gregata. The disease is a late-season, cool-temperature,soilborne fungus which in appropriate favorable weather can cause up to30 percent yield losses in soybean fields. BSR information is gatheredin a greenhouse with a plant in a pot then a root dip procedure isemployed. BSR_R is an opportunistic field rating scale is 1-9.Sudden Death Syndrome (SDS or SDS_R) This disease is caused byslow-growing strains of Fursarium solani that produce bluish pigments inculture. The disease is a mid to late season, soil borne disease thatoccurs in soybean fields with high yield potential. Yield losses may betotal or severe in infected fields. Sudden Death Syndrome (SDS_R) isbased on leaf area affected. The scale used for these tests is 1-5 or ifidentified as SDS_R the scale is 1-9.Sclerotinia White Mold (SCL_R) This disease is caused by the fungalpathogen Sclerotinia sclerotium. The fungus can overwinter in the soilfor many years as sclerotia and infecting plants in prolonged periods ofhigh humidity or rainfall. Yield losses may be total or severe ininfected fields. Sclerotinia White Mold (SCL_R) rating is a field rating(1-9 scale) based the percentage of wilting of dead plants in a plot.Frog Eye Leaf Spot (FELSR) This is caused by the fungal pathogenCercospora sojina. The fungus survives as mycelium in infected seeds andin infested debris. With adequate moisture new leaves become infected asthey develop until all the leaves are infected. Yield losses may be upto 15% in severe infected fields. Frog Eye Leaf Spot (FELSR) rating is afield rating (1-9 scale) based the percentage of leaf area affected.Soybean Cyst Nematode (SCN) The Soybean Cyst Nematode Heteroderaglycines, is a small plant-parasitic roundworm that attacks the roots ofsoybeans. Soybean Cyst Nematode (SCN) for purposes of these tests isdone as a greenhouse screen—30 day screen using infested soil. Therating scale is based upon female reproduction index on a susceptiblecheck set where <10%=R (RESISTANT); <30%=MR (MODERATELY RESISTANT);<60%=MS (MODERATELY SUSPECTIBLE); >60%=S (SUSPECTIBLE). In priorityorder, the screening races include: 3, 14, & 1.Maturity Date (MAT or MRTYN) Plants are considered mature when 95% ofthe pods have reached their mature color. For MAT, the number of days iscalculated from August 31 or MRTYN is calculated from the number of daysfrom planting date.Relative Maturity Group (RM) Industry Standard for varieties groups,based day length or latitude. Long day length (northern areas in theNorthern Hemisphere) is classified as (Groups 000, 00, 0,). Mid daylengths variety groups lie in the middle (Groups I-VI). Very short daylengths variety groups (southern areas in Northern Hemisphere) areclassified as (Groups VII, VIII, IX).Seed Yield (YSGMN) The actual grain yield at harvest reported in theunits bushels/acre.Shattering (SHAT or STR_R) The rate of pod dehiscence prior to harvest.Pod dehiscence is the process of beans dropping out of the pods. Shatter(SHAT) for these tests the rating of pre-harvest loses is based onamount of plants with open pods on a scale of 1-5, STR_R on a scale 1-9.Plant Means the plant, the plant's cells, plant protoplasts, plant cellsof tissue culture from which soybean plants can be regenerated, plantcalli, plant clumps, and plant cells that are intact in plants or partsof the plants, such as pollen, nodes, roots, flowers, seeds, pods,leaves, stems, pod and the like.

Definitions of Staging of Development

The plant development staging system employed in the testing of thisinvention divides stages as vegetative (V) and reproductive (R). Thissystem accurately identifies the stages of any soybean plant. However,all plants in a given field will not be in the stage at the same time.Therefore, each specific V or R stage is defined as existing when 50% ormore of the plants in the field are in or beyond that stage.

The first two stages of V are designated a VE (emergence) and VC(cotyledon stage). Subdivisions of the V stages are then designatednumerically as V1, V2, V3 through V (n). The last V stage is designatedas V (n), where (n) represents the number for the last node stage of thespecific variety. The (n) will vary with variety and environment. Theeight subdivisions of the reproductive stages (R) states are alsodesignated numerically. R1=beginning bloom; R2=full bloom; R3=beginningpod; R4=full pod; R5=beginning seed; R6=full seed; R7=beginningmaturity; R8=full maturity.

Soybean Cultivar

The present invention is S06-01JR122235 is a Group IV Maturity soybeancultivar. This cultivar is developed for use of the beans.S06-01JR122235 has an area of best adaptation which occurs within thelower Midwest where other mid Maturity Group IV soybeans are grown. Thepresent invention is especially adapted to regions where there isinfestation by Soybean Cyst Nematode.

This soybean variety in one embodiment carries one or more transgenes,for example, the glyphosate tolerance transgene, a desaturase gene orother transgenes. In another embodiment of the invention does not carryany herbicide resistance traits. In yet another embodiment of theinvention, the soybean does not carry any transgenes but carries allelesfor aphid resistance, cyst nematode resistance and/or brown stem rot orthe like.

The traits of the invention are listed below.

TRAITS

Plant Characteristics RR ® Y STS ® N Flower Color P Pubescence Color TPod Color B Hilum Color BL % Protein 13% mst. 34.8 % Oil @ 13% mst. 19.1Seed Size/Lb 2800.0 Stem Termination Indeterminate

Plant Health Rps Gene 1C Phytophthora Root Rot Tolerance 4.6 SCN RACE 1FI % SCN RACE 3 FI % 1.0 SCN RACE 5 FI % SCN RACE 14 FI % 19.0 Root KnotNematode - Incognita Root Knot Nematode - Arenaria Stem Canker(Southern) 1.0 Stem Canker Tolerance (Southern) Rps gene indicates thespecific gene for resistance but if none are indicated then none areknown to be present Y = Yes, has trait. N = no does not contain traitSCN = Soybean Cyst Nematode RoundUp Ready ®, Roundup ® and Roundup ®Ultra are trademarks of Monsanto Company. STS ® is a trademark ofDuPont. Ratings are on a 1 to 9 scale with 1 being the best.

Additional traits Leaf Shape - Ovate Hypocotyl Length: Long StemTermination: Indeterminate Powdery Mildew: Resistant Seed Coat Color:Yellow Sudden Death Syndrome - Mod. Hypocotyl color: Dark Purple SCNresistance - R*3, R*14 Maturity Group - 4 *R = race Relative Maturity -4-4 Phytophthora sojae R*1, 3, 7 = Resistant and 4 = S* Seed CoatPeroxidase: unknown S* = Susceptible Frogeye Leaf Spot - Resistant

The present invention provides methods and composition relating toplants, seeds and derivatives of the soybean cultivar S06-01JR122235.Soybean cultivar S06-01JR122235 has superior characteristics. TheS06-01JR122235 line has been selfed sufficient number of generations toprovide a stable and uniform plant variety.

Cultivar S06-01JR122235 shows no variants other than expected due toenvironment or that normally would occur for almost any characteristicduring the course of repeated sexual reproduction. Some of the criteriaused to select in various generations include: seed yield, emergence,appearance, disease tolerance, maturity, plant height, and shatteringdata.

The inventor believes that S06-01JR122235 is similar to the comparisonvarieties. However, as shown in the tables, S06-01JR122235 differs fromthese cultivars.

Direct comparisons were made between S06-01JR122235 and the listedcommercial varieties. Traits measured included yield, maturity, lodging,plant height, branching, field emergence, and shatter. The results ofthe comparison are presented in below. The number of tests in which thevarieties were compared is shown with the environments, mean andstandard deviation for some traits.

The present invention S06-01JR122235 can carry genetic engineeredrecombinant genetic material to give improved traits or qualities to thesoybean. For example, but not limited to, the present invention cancarry the glyphosate resistance gene for herbicide resistance as taughtin the Monsanto patents (WO92/00377, WO92/04449, U.S. Pat. No. 5,188,642and U.S. Pat. No. 5,312,910) or STS mutation for herbicide resistance.Additional traits carried in transgenes or mutation can be transferredinto the present invention. Some of these genes include genes that givedisease resistance to sclerotinia such as the oxalate oxidase (Ox Ox)gene as taught in PCT/FR92/00195 Rhone Polunc and/or an oxalatedecarboxylase gene for disease resistance or genes designed to alter thesoybean oil within the seed such as desaturase, thioesterase genes(shown in EP0472722, U.S. Pat. No. 5,344,771) or genes designed to alterthe soybean's amino acid characteristics. This line can be crossed withanother soybean line which carries a gene that acts to provide herbicideresistance or alter the saturated and/or unsaturated fatty acid contentof the oil within the seed, or the amino acid profile of the seed.

The present invention S06-01JR122235 is employed in a number of plotrepetitions to establish trait characteristics.

Geographic Summary

The target variety yield is given as a percent of the trial average atall locations shown in FIG. 1 and each geographic segment West to Eastor Central (Cntrl) to South East where there are three or morelocations. The plots for these trials are two row 17.5 foot plotsplanted in 30-inch row spacing. The plants in the plots are acombination of experimental material and commercial material. There areusually 36 varieties and there are approximately 300 plants of eachvariety with two replications in about 20-25 locations. The data in FIG.1 is only charted if there are at least 3 or more locations. N>=3

The present invention differs from the mean of the comparison commercialand experimental soybean lines in that the present soybean cultivar isacross 50 locations, not quite meeting the mean yield of the group ofsoybeans (GRP_MN) that were tested and displayed in this geographicsegment chart (GSEGC). When just the Mid South and Central regions arereviewed the testing data for the present invention is yielding about0.7% above the group mean in these regions. However, this invention, inthe Delta and South East regions, is not giving the best yieldperformance as it is yielding 5.0%to 5.5% below the Group Mean yields inthese regions.

The present invention S06-01JR122235 is employed in a trial with anumber of environments. The results of the grain yield at standardmoisture are shown in the chart as shown in FIG. 2. The presentinvention is a variety that will yield at the mean or slightly above inthe mid high and high yielding environments. If the environment isextremely low yielding the present invention tends to under perform whencompared to the group mean. At about the 47-50 bushels per acre rangethe present invention starts to accelerate its yield and meet the levelof the group mean. There are a number of outlying results which makesthe yield stability of the present invention slightly less than thegroup mean stability in these locations. These tests allow theusefulness of the invention to be shown in light of the environmentalgenetic interactions.

2004-05 Research Data Yield Performance Chart VHNO YGSMN MRTYN HLDGRPLHTN PRR_R IC_(——)R SDS_R SCL_R FELSR STR_R GLDGR PLBRR EMRGRCommercial 55.5 138.2 4.3 98.0 4.2 7.4 3.1 5.6 7.0 1.1 4.6 4.4 3.1 1S43-B1 54.3 135.7 4.3 92.9 3.9 4.0 3.0 6.4 2.5 2.0 5.1 5.5 3.3Commercial 54.0 133.2 4.1 94.7 4.1 5.9 3.9 4.1 2.5 3.6 6.2 4.0 3.2 201JR122235 53.2 136.9 3.9 97.6 4.1 6.4 2.9 4.0 3.0 3.4 4.3 5.5 3.1Environ- 50.0 23.0 28.0 28.0 2.0 2.0 12.0 1.0 1.0 2.0 2.0 2.0 12.0 mentsMean 54.3 136.0 4.2 95.8 4.0 5.9 3.2 5.0 3.8 2.5 5.1 4.9 3.2 LSD (0.05)1.3 2.5 0.6

Each of these lines has their own positive traits. Each of these linesis different from the present invention. The present invention has abetter rating for Sudden Death Syndrome than does the mean for thecommercial lines to which it is compared. The present invention also hasbetter ratings for other plant diseases such as Sclerotinia White Moldand Frogeye Leaf Spot than the mean for the tested material. The presentinvention has certain positive agronomic traits shown by its rating forharvest lodging which is better than the mean. However, the presentinvention is not showing as much yield as the mean nor does it have theshatter rating of the mean of the tested material. The yield and otherdata is a snapshot of each of these lines' results in the specificenvironment and will differ when other environmental interactions aremeasured.

This invention also is directed to methods for producing a new soybeanplant by crossing a first parent plant with a second parent plantwherein the first or second parent plant is the present invention.Additionally, the present invention may be used in the varietydevelopment process to derive progeny in a breeding population orcrossing. Further, both first and second parent plants can come from thesoybean line S06-01JR122235. A variety of breeding methods can beselected depending on the mode of reproduction, the trait, the conditionof the germplasm. Thus, any such methods using the S06-01JR122235 arepart of this invention: selfing, backcrosses, recurrent selection, massselection and the like.

The scope of the present invention includes any use on S06-01JR122235 ofmarker methods. Through the use of markers such as SSRs, RFLP's, SNPs,Ests, AFLPs, gene primers, and the like to identify genetic alleleswhich can be identified and breed with marker assistance into thepresent invention with little to no superfluous germplasm being draggedinto the present invention. This results in formation of the presentinvention plus for example, cyst nematode resistance, brown stem rotresistance, aphid resistance, Phytophthora resistance, IDC resistance,BT genes or male sterility genes or glyphosate tolerance genes ordrought tolerance genes or alleles and the like. Transgenes maybedirectly introduced into the cultivar using genetic engineering andtransformation techniques well known in the art, some of which aredescribed above, or are originally introduced into a donor, parent usinggenetic engineering and transformation techniques, and using the donorin a marker assisted trait conversion process, the trait is moved forexample by backcrossing. A transgene typically comprises a nucleotidesequence whose expression is responsible or contributes to the trait,under the control of a promoter capable of directing the expression ofthe nucleotide sequence at the desired time in the desired tissue orpart of the plant. Constitutive, tissue-specific or inducible promotersall have different purposes and each could be employed. The transgenemay also comprise other regulatory elements such as for exampletranslation enhancers or termination signals. The transgene may beadapted to be transcribed and translated into a protein, or to encodeRNA in a sense or antisense orientation such that it is not translatedor only partially translated.

The scope of the present invention also includes any use onS06-01JR122235 of transformation methods. Transformation methods aremeans for integrating new genetic coding sequences (transgenes) into theplant's genome by the incorporation of these sequences into a plantthrough man's assistance. Many dicots including soybeans can easily betransformed with Agrobacterium. Methods of introducing desiredrecombinant DNA molecule into plant tissue include the direct infectionor co-cultivation of plant cells with Agrobacterium tumefaciens, Horschet al., Science, 227:1229 (1985). Descriptions of Agrobacterium vectorsystems and methods are shown in Gruber, et al., “Vectors for PlantTransformation, in Methods in Plant Molecular Biology & Biotechnology”in Glich et al., (Eds. pp. 89-119, CRC Press, 1993). Transformed plantsobtained via protoplast transformation are also intended to be withinthe scope of this invention. The most common method of transformationafter the use of agrobacterium is referred to as gunning ormicroprojectile bombardment. This process has small gold-coatedparticles coated with DNA (including the transgene) shot into thetransformable material. Techniques for gunning DNA into cells, tissue,explants, meristems, callus, embryos, and the like are well known in theprior art. The DNA used for transformation of these plants clearly maybe circular, linear, and double or single stranded. Usually, the DNA isin the form of a plasmid. The plasmid usually contains regulatory and/ortargeting sequences which assists the expression of the gene in theplant. The methods of forming plasmids for transformation are known inthe art. Plasmid components can include such items as: leader sequences,transit polypeptides, promoters, terminators, genes, introns, markergenes, etc. The structures of the gene orientations can be sense,antisense, partial antisense, or partial sense: multiple gene copies canbe used.

After the transformation of the plant material is complete, the nextstep is identifying the cells or material, which has been transformed.In some cases, a screenable marker is employed such as thebeta-glucuronidase gene of the uidA locus of E. Coli. Then, thetransformed cells expressing the colored protein are selected for eitherregeneration or further use. In many cases, a selectable markeridentifies the transformed material. The putatively transformed materialis exposed to a toxic agent at varying concentrations. The cells nottransformed with the selectable marker, which provides resistance tothis toxic agent, die. Cells or tissues containing the resistantselectable marker generally proliferate. It has been noted that althoughselectable markers protect the cells from some of the toxic affects ofthe herbicide or antibiotic, the cells may still be slightly affected bythe toxic agent by having slower growth rates. If the transformedmaterials are cell lines then these lines are used to regenerate plants.The cells' lines are treated to induce tissue differentiation. Methodsof regeneration of plants from cellular material are well known in theart. The plants from the transformation process or the plants resultingfrom a cross using a transformed line or the progeny of such plants aretransgenic plants that carry the transgene.

DEPOSIT INFORMATION

Applicants have made a deposit of at least 2500 seeds of soybeancultivar S06-01JR122235 with the American Type Culture Collection(ATCC), Manassas, Va. 20110 on Sep. 12, 2007 and designated PTA-8626.The seeds were tested on Sep. 25, 2007 and found to be viable. Access tothis deposit will be available during the pendency of the application tothe Commissioner for Patents and persons determined by the Commissionerto be entitled thereto upon request. Upon granting of a patent on anyclaims in the application, the Applicants will make the depositavailable to the public pursuant to 37 CFR §1.808. Additionally,Applicants will meet the requirements of 37 CFR §1.801-1.809, includingproviding an indication of the viability of the sample when the depositis made. The ATCC deposit will be maintained in that depository, whichis a public depository, for a period of 30 years, or 5 years after thelast request, or for the effective life of the patent, whichever islonger, and will be replaced if it becomes nonviable during that period.

Accordingly, the present invention has been described with some degreeof particularity directed to the preferred embodiment of the presentinvention. It should be appreciated, though, that the present inventionis defined by the following claims construed in light of the prior artso that modifications or changes may be made to the preferred embodimentof the present invention without departing from the inventive conceptscontained herein.

1. A soybean seed designated S06-01JR122235, a sample of said seeddeposited under ATCC Accession No. PTA-8626.
 2. A plant, or partsthereof, produced by growing the seed of claim
 1. 3. Pollen of the plantof claim
 2. 4. A soybean plant, or parts thereof, having all of thephysiological and morphological characteristics of the soybean plant ofclaim
 2. 5. A tissue culture of regenerable cells of the soybean plantof claim
 2. 6. The tissue culture according to claim 5, wherein thecells are obtained from the group consisting of leaf, pollen, embryo,meristematic cell, root, root tip, anther, stomatal cell, flower, seed,stem and pod.
 7. A soybean plant regenerated from the tissue culture ofclaim 6, having all of the morphological and physiologicalcharacteristics of soybean cultivar S06-01JR122235.
 8. A method forproducing a soybean seed comprising crossing two soybean plants andharvesting the resultant soybean seed, wherein at least one soybeanplant is the soybean plant of claim
 2. 9. A method for producing ahybrid soybean seed comprising crossing the soybean plant according toclaim 2 with a second soybean plant and harvesting the resultant hybridsoybean seed.
 10. A method for producing a S06-01JR122235-derivedsoybean plant, comprising: a) crossing soybean line S06-01JR122235, asample of said line deposited under ATCC Accession No. PTA-8626, with asecond soybean plant to yield progeny soybean seed; and b) growing saidprogeny soybean seed to yield said S06-01JR122235-derived soybean plant.11. The method of claim 8, wherein the second soybean plant istransgenic.
 12. The method of claim 11 wherein the transgenic soybeanplant contains genetic material conferring a trait selected from thegroup consisting of herbicide resistance, nematode resistance, insectresistance, resistance to disease, and male sterility.
 13. The method ofclaim 12 wherein the resistance to disease is through an oxalate oxidaseencoding polynucleotide sequence or an oxalate decarboxylase encodingpolynucleotide sequence.